S protein of severe acute respiratory syndrome-associated coronavirus mediates entry into hepatoma cell lines and is targeted by neutralizing antibodies in infected patients
Identifieur interne : 005A72 ( Main/Exploration ); précédent : 005A71; suivant : 005A73S protein of severe acute respiratory syndrome-associated coronavirus mediates entry into hepatoma cell lines and is targeted by neutralizing antibodies in infected patients
Auteurs : Heike Hofmann [Allemagne] ; Kim Hattermann [Allemagne] ; Andrea Marzi [Allemagne] ; Thomas Gramberg [Allemagne] ; Martina Geier [Allemagne] ; Mandy Krumbiegel [Allemagne] ; Seraphin Kuate [Allemagne] ; Klaus Überla [Allemagne] ; Matthias Niedrig [Allemagne] ; Stefan Pöhlmann [Allemagne]Source :
- Journal of virology [ 0022-538X ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (sang), Carcinome hépatocellulaire, Foie (cytologie), Foie (virologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Humains, Lapins, Lentivirus (génétique), Lignée cellulaire, Lignée cellulaire tumorale, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Rein (cytologie), Rein (virologie), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Tests de neutralisation, Transfection, Tumeurs du foie, Virion, Virus du SRAS (pathogénicité).
- MESH :
- cytologie : Foie, Rein.
- génétique : Glycoprotéines membranaires, Lentivirus, Protéines de l'enveloppe virale.
- immunologie : Anticorps antiviraux, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- pathogénicité : Virus du SRAS.
- sang : Anticorps antiviraux.
- virologie : Foie, Rein, Syndrome respiratoire aigu sévère.
- Pascal (Inist)
- Animaux, Carcinome hépatocellulaire, Coronavirus, Glycoprotéine de spicule des coronavirus, Homme, Humains, Lapins, Lignée cellulaire, Lignée cellulaire tumorale, Tests de neutralisation, Transfection, Tumeurs du foie, Virion, Vitronectine, Protéine, Lignée cellulaire, Cellule cible, Anticorps neutralisant, Neutralisation, Infection, Microbiologie, Syndrome respiratoire aigu sévère, Carcinome hépatocellulaire, Virologie.
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Antibodies, Viral (immunology), Carcinoma, Hepatocellular, Cell Line, Cell Line, Tumor, Cell line, Coronavirus, Hepatocellular carcinoma, Human, Humans, Infection, Kidney (cytology), Kidney (virology), Lentivirus (genetics), Liver (cytology), Liver (virology), Liver Neoplasms, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Microbiology, Neutralization, Neutralization Tests, Neutralizing antibody, Protein, Rabbits, SARS Virus (pathogenicity), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome, Spike Glycoprotein, Coronavirus, Target cell, Transfection, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism), Virion, Virology, Vitronectin.
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- cytology : Kidney, Liver.
- genetics : Lentivirus.
- immunology : Severe Acute Respiratory Syndrome.
- chemical , metabolism : Membrane Glycoproteins, Viral Envelope Proteins.
- pathogenicity : SARS Virus.
- virology : Kidney, Liver, Severe Acute Respiratory Syndrome.
- Animals, Carcinoma, Hepatocellular, Cell Line, Cell Line, Tumor, Humans, Liver Neoplasms, Neutralization Tests, Rabbits, Spike Glycoprotein, Coronavirus, Transfection, Virion.
Abstract
The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) causes severe pneumonia with a fatal outcome in approximately 10% of patients. SARS-CoV is not closely related to other coronaviruses but shares a similar genome organization. Entry of coronaviruses into target cells is mediated by the viral S protein. We functionally analyzed SARS-CoV S using pseudotyped lentiviral particles (pseudotypes). The SARS-CoV S protein was found to be expressed at the cell surface upon transient transfection. Coexpression of SARS-CoV S with human immunodeficiency virus-based reporter constructs yielded viruses that were infectious for a range of cell lines. Most notably, viral pseudotypes harboring SARS-CoV S infected hepatoma cell lines but not T- and B-cell lines. Infection of the hepatoma cell line Huh-7 was also observed with replication-competent SARS-CoV, indicating that hepatocytes might be targeted by SARS-CoV in vivo. Inhibition of vacuolar acidification impaired infection by SARS-CoV S-bearing pseudotypes, indicating that S-mediated entry requires low pH. Finally, infection by SARS-CoV S pseudotypes but not by vesicular stomatitis virus G pseudotypes was efficiently inhibited by a rabbit serum raised against SARS-CoV particles and by sera from SARS patients, demonstrating that SARS-CoV S is a target for neutralizing antibodies and that such antibodies are generated in SARS-CoV-infected patients. Our results show that viral pseudotyping can be employed for the analysis of SARS-CoV S function. Moreover, we provide evidence that SARS-CoV infection might not be limited to lung tissue and can be inhibited by the humoral immune response in infected patients.
Url:
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000876
- to stream PascalFrancis, to step Curation: 000114
- to stream PascalFrancis, to step Checkpoint: 000778
- to stream Main, to step Merge: 005F29
- to stream Pmc, to step Corpus: 000679
- to stream Pmc, to step Curation: 000679
- to stream Pmc, to step Checkpoint: 001535
- to stream PubMed, to step Corpus: 002D76
- to stream PubMed, to step Curation: 002D76
- to stream PubMed, to step Checkpoint: 002B00
- to stream Ncbi, to step Merge: 000834
- to stream Ncbi, to step Curation: 000834
- to stream Ncbi, to step Checkpoint: 000834
- to stream Main, to step Merge: 005530
- to stream Main, to step Curation: 005A72
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">S protein of severe acute respiratory syndrome-associated coronavirus mediates entry into hepatoma cell lines and is targeted by neutralizing antibodies in infected patients</title><author><name sortKey="Hofmann, Heike" sort="Hofmann, Heike" uniqKey="Hofmann H" first="Heike" last="Hofmann">Heike Hofmann</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation></author><author><name sortKey="Hattermann, Kim" sort="Hattermann, Kim" uniqKey="Hattermann K" first="Kim" last="Hattermann">Kim Hattermann</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Robert Koch Institute</s1><s2>13353 Berlin</s2><s3>DEU</s3><sZ>2 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Marzi, Andrea" sort="Marzi, Andrea" uniqKey="Marzi A" first="Andrea" last="Marzi">Andrea Marzi</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation></author><author><name sortKey="Gramberg, Thomas" sort="Gramberg, Thomas" uniqKey="Gramberg T" first="Thomas" last="Gramberg">Thomas Gramberg</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation></author><author><name sortKey="Geier, Martina" sort="Geier, Martina" uniqKey="Geier M" first="Martina" last="Geier">Martina Geier</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation></author><author><name sortKey="Krumbiegel, Mandy" sort="Krumbiegel, Mandy" uniqKey="Krumbiegel M" first="Mandy" last="Krumbiegel">Mandy Krumbiegel</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation></author><author><name sortKey="Kuate, Seraphin" sort="Kuate, Seraphin" uniqKey="Kuate S" first="Seraphin" last="Kuate">Seraphin Kuate</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department for Molecular and Medical Virology, Ruhr University Bochum</s1><s2>44801 Bochum</s2><s3>DEU</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>44801 Bochum</wicri:noRegion><wicri:noRegion>Ruhr University Bochum</wicri:noRegion><wicri:noRegion>44801 Bochum</wicri:noRegion></affiliation></author><author><name sortKey="Uberla, Klaus" sort="Uberla, Klaus" uniqKey="Uberla K" first="Klaus" last="Überla">Klaus Überla</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department for Molecular and Medical Virology, Ruhr University Bochum</s1><s2>44801 Bochum</s2><s3>DEU</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>44801 Bochum</wicri:noRegion><wicri:noRegion>Ruhr University Bochum</wicri:noRegion><wicri:noRegion>44801 Bochum</wicri:noRegion></affiliation></author><author><name sortKey="Niedrig, Matthias" sort="Niedrig, Matthias" uniqKey="Niedrig M" first="Matthias" last="Niedrig">Matthias Niedrig</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Robert Koch Institute</s1><s2>13353 Berlin</s2><s3>DEU</s3><sZ>2 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Pohlmann, Stefan" sort="Pohlmann, Stefan" uniqKey="Pohlmann S" first="Stefan" last="Pöhlmann">Stefan Pöhlmann</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">04-0316775</idno><date when="2004">2004</date><idno type="stanalyst">PASCAL 04-0316775 INIST</idno><idno type="RBID">Pascal:04-0316775</idno><idno type="wicri:Area/PascalFrancis/Corpus">000876</idno><idno type="wicri:Area/PascalFrancis/Curation">000114</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000778</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000778</idno><idno type="wicri:doubleKey">0022-538X:2004:Hofmann H:s:protein:of</idno><idno type="wicri:Area/Main/Merge">005F29</idno><idno type="wicri:source">PMC</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC416513</idno><idno type="RBID">PMC:416513</idno><idno type="wicri:Area/Pmc/Corpus">000679</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000679</idno><idno type="wicri:Area/Pmc/Curation">000679</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000679</idno><idno type="wicri:Area/Pmc/Checkpoint">001535</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001535</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:15163706</idno><idno type="wicri:Area/PubMed/Corpus">002D76</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002D76</idno><idno type="wicri:Area/PubMed/Curation">002D76</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002D76</idno><idno type="wicri:Area/PubMed/Checkpoint">002B00</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002B00</idno><idno type="wicri:Area/Ncbi/Merge">000834</idno><idno type="wicri:Area/Ncbi/Curation">000834</idno><idno type="wicri:Area/Ncbi/Checkpoint">000834</idno><idno type="wicri:doubleKey">0022-538X:2004:Hofmann H:s:protein:of</idno><idno type="wicri:Area/Main/Merge">005530</idno><idno type="wicri:Area/Main/Curation">005A72</idno><idno type="wicri:Area/Main/Exploration">005A72</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">S protein of severe acute respiratory syndrome-associated coronavirus mediates entry into hepatoma cell lines and is targeted by neutralizing antibodies in infected patients</title><author><name sortKey="Hofmann, Heike" sort="Hofmann, Heike" uniqKey="Hofmann H" first="Heike" last="Hofmann">Heike Hofmann</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation></author><author><name sortKey="Hattermann, Kim" sort="Hattermann, Kim" uniqKey="Hattermann K" first="Kim" last="Hattermann">Kim Hattermann</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Robert Koch Institute</s1><s2>13353 Berlin</s2><s3>DEU</s3><sZ>2 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Marzi, Andrea" sort="Marzi, Andrea" uniqKey="Marzi A" first="Andrea" last="Marzi">Andrea Marzi</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation></author><author><name sortKey="Gramberg, Thomas" sort="Gramberg, Thomas" uniqKey="Gramberg T" first="Thomas" last="Gramberg">Thomas Gramberg</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation></author><author><name sortKey="Geier, Martina" sort="Geier, Martina" uniqKey="Geier M" first="Martina" last="Geier">Martina Geier</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation></author><author><name sortKey="Krumbiegel, Mandy" sort="Krumbiegel, Mandy" uniqKey="Krumbiegel M" first="Mandy" last="Krumbiegel">Mandy Krumbiegel</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation></author><author><name sortKey="Kuate, Seraphin" sort="Kuate, Seraphin" uniqKey="Kuate S" first="Seraphin" last="Kuate">Seraphin Kuate</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department for Molecular and Medical Virology, Ruhr University Bochum</s1><s2>44801 Bochum</s2><s3>DEU</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>44801 Bochum</wicri:noRegion><wicri:noRegion>Ruhr University Bochum</wicri:noRegion><wicri:noRegion>44801 Bochum</wicri:noRegion></affiliation></author><author><name sortKey="Uberla, Klaus" sort="Uberla, Klaus" uniqKey="Uberla K" first="Klaus" last="Überla">Klaus Überla</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department for Molecular and Medical Virology, Ruhr University Bochum</s1><s2>44801 Bochum</s2><s3>DEU</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>44801 Bochum</wicri:noRegion><wicri:noRegion>Ruhr University Bochum</wicri:noRegion><wicri:noRegion>44801 Bochum</wicri:noRegion></affiliation></author><author><name sortKey="Niedrig, Matthias" sort="Niedrig, Matthias" uniqKey="Niedrig M" first="Matthias" last="Niedrig">Matthias Niedrig</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Robert Koch Institute</s1><s2>13353 Berlin</s2><s3>DEU</s3><sZ>2 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Pohlmann, Stefan" sort="Pohlmann, Stefan" uniqKey="Pohlmann S" first="Stefan" last="Pöhlmann">Stefan Pöhlmann</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg</s1><s2>91054 Erlangen</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>91054 Erlangen</wicri:noRegion><placeName><settlement type="city">Erlangen</settlement><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Moyenne-Franconie</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Antibodies, Viral (immunology)</term><term>Carcinoma, Hepatocellular</term><term>Cell Line</term><term>Cell Line, Tumor</term><term>Cell line</term><term>Coronavirus</term><term>Hepatocellular carcinoma</term><term>Human</term><term>Humans</term><term>Infection</term><term>Kidney (cytology)</term><term>Kidney (virology)</term><term>Lentivirus (genetics)</term><term>Liver (cytology)</term><term>Liver (virology)</term><term>Liver Neoplasms</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Membrane Glycoproteins (metabolism)</term><term>Microbiology</term><term>Neutralization</term><term>Neutralization Tests</term><term>Neutralizing antibody</term><term>Protein</term><term>Rabbits</term><term>SARS Virus (pathogenicity)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Severe acute respiratory syndrome</term><term>Spike Glycoprotein, Coronavirus</term><term>Target cell</term><term>Transfection</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Envelope Proteins (metabolism)</term><term>Virion</term><term>Virology</term><term>Vitronectin</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps antiviraux (sang)</term><term>Carcinome hépatocellulaire</term><term>Foie (cytologie)</term><term>Foie (virologie)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Lapins</term><term>Lentivirus (génétique)</term><term>Lignée cellulaire</term><term>Lignée cellulaire tumorale</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Rein (cytologie)</term><term>Rein (virologie)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Tests de neutralisation</term><term>Transfection</term><term>Tumeurs du foie</term><term>Virion</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Foie</term><term>Rein</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Kidney</term><term>Liver</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lentivirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Lentivirus</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Foie</term><term>Rein</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Kidney</term><term>Liver</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Carcinoma, Hepatocellular</term><term>Cell Line</term><term>Cell Line, Tumor</term><term>Humans</term><term>Liver Neoplasms</term><term>Neutralization Tests</term><term>Rabbits</term><term>Spike Glycoprotein, Coronavirus</term><term>Transfection</term><term>Virion</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term><term>Carcinome hépatocellulaire</term><term>Coronavirus</term><term>Glycoprotéine de spicule des coronavirus</term><term>Homme</term><term>Humains</term><term>Lapins</term><term>Lignée cellulaire</term><term>Lignée cellulaire tumorale</term><term>Tests de neutralisation</term><term>Transfection</term><term>Tumeurs du foie</term><term>Virion</term><term>Vitronectine</term><term>Protéine</term><term>Lignée cellulaire</term><term>Cellule cible</term><term>Anticorps neutralisant</term><term>Neutralisation</term><term>Infection</term><term>Microbiologie</term><term>Syndrome respiratoire aigu sévère</term><term>Carcinome hépatocellulaire</term><term>Virologie</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) causes severe pneumonia with a fatal outcome in approximately 10% of patients. SARS-CoV is not closely related to other coronaviruses but shares a similar genome organization. Entry of coronaviruses into target cells is mediated by the viral S protein. We functionally analyzed SARS-CoV S using pseudotyped lentiviral particles (pseudotypes). The SARS-CoV S protein was found to be expressed at the cell surface upon transient transfection. Coexpression of SARS-CoV S with human immunodeficiency virus-based reporter constructs yielded viruses that were infectious for a range of cell lines. Most notably, viral pseudotypes harboring SARS-CoV S infected hepatoma cell lines but not T- and B-cell lines. Infection of the hepatoma cell line Huh-7 was also observed with replication-competent SARS-CoV, indicating that hepatocytes might be targeted by SARS-CoV in vivo. Inhibition of vacuolar acidification impaired infection by SARS-CoV S-bearing pseudotypes, indicating that S-mediated entry requires low pH. Finally, infection by SARS-CoV S pseudotypes but not by vesicular stomatitis virus G pseudotypes was efficiently inhibited by a rabbit serum raised against SARS-CoV particles and by sera from SARS patients, demonstrating that SARS-CoV S is a target for neutralizing antibodies and that such antibodies are generated in SARS-CoV-infected patients. Our results show that viral pseudotyping can be employed for the analysis of SARS-CoV S function. Moreover, we provide evidence that SARS-CoV infection might not be limited to lung tissue and can be inhibited by the humoral immune response in infected patients.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Bavière</li><li>Berlin</li><li>District de Moyenne-Franconie</li></region><settlement><li>Berlin</li><li>Erlangen</li></settlement></list><tree><country name="Allemagne"><region name="Bavière"><name sortKey="Hofmann, Heike" sort="Hofmann, Heike" uniqKey="Hofmann H" first="Heike" last="Hofmann">Heike Hofmann</name></region><name sortKey="Geier, Martina" sort="Geier, Martina" uniqKey="Geier M" first="Martina" last="Geier">Martina Geier</name><name sortKey="Geier, Martina" sort="Geier, Martina" uniqKey="Geier M" first="Martina" last="Geier">Martina Geier</name><name sortKey="Gramberg, Thomas" sort="Gramberg, Thomas" uniqKey="Gramberg T" first="Thomas" last="Gramberg">Thomas Gramberg</name><name sortKey="Gramberg, Thomas" sort="Gramberg, Thomas" uniqKey="Gramberg T" first="Thomas" last="Gramberg">Thomas Gramberg</name><name sortKey="Hattermann, Kim" sort="Hattermann, Kim" uniqKey="Hattermann K" first="Kim" last="Hattermann">Kim Hattermann</name><name sortKey="Hofmann, Heike" sort="Hofmann, Heike" uniqKey="Hofmann H" first="Heike" last="Hofmann">Heike Hofmann</name><name sortKey="Krumbiegel, Mandy" sort="Krumbiegel, Mandy" uniqKey="Krumbiegel M" first="Mandy" last="Krumbiegel">Mandy Krumbiegel</name><name sortKey="Krumbiegel, Mandy" sort="Krumbiegel, Mandy" uniqKey="Krumbiegel M" first="Mandy" last="Krumbiegel">Mandy Krumbiegel</name><name sortKey="Kuate, Seraphin" sort="Kuate, Seraphin" uniqKey="Kuate S" first="Seraphin" last="Kuate">Seraphin Kuate</name><name sortKey="Marzi, Andrea" sort="Marzi, Andrea" uniqKey="Marzi A" first="Andrea" last="Marzi">Andrea Marzi</name><name sortKey="Marzi, Andrea" sort="Marzi, Andrea" uniqKey="Marzi A" first="Andrea" last="Marzi">Andrea Marzi</name><name sortKey="Niedrig, Matthias" sort="Niedrig, Matthias" uniqKey="Niedrig M" first="Matthias" last="Niedrig">Matthias Niedrig</name><name sortKey="Pohlmann, Stefan" sort="Pohlmann, Stefan" uniqKey="Pohlmann S" first="Stefan" last="Pöhlmann">Stefan Pöhlmann</name><name sortKey="Pohlmann, Stefan" sort="Pohlmann, Stefan" uniqKey="Pohlmann S" first="Stefan" last="Pöhlmann">Stefan Pöhlmann</name><name sortKey="Uberla, Klaus" sort="Uberla, Klaus" uniqKey="Uberla K" first="Klaus" last="Überla">Klaus Überla</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005A72 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005A72 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:04-0316775
|texte= S protein of severe acute respiratory syndrome-associated coronavirus mediates entry into hepatoma cell lines and is targeted by neutralizing antibodies in infected patients
}}
| This area was generated with Dilib version V0.6.33. |  |